Evaluation of Corpus Luteum Vascularization in Recipient Mares by Using Color Doppler Ultrasound

Background: Embryo transfer is one of the most commonly used reproductive biotechnique. The success embryo also affected by synchrony estrus and ovulation between donor recipient animals. In horse reproduction, ultrasonography has been used, among other purposes, to diagnose early pregnancy. However, only color Doppler imaging mode makes it possible evaluate vascular architecture hemodynamic aspects vessels in several organs, especially corpus luteum. objective this study was evaluate, based on ultrasound, luteum vascularization function from mares at timing.Materials, Methods & Results: Mangalarga Machador 5 10-year-old a range live weights 350 450 kg were for experiment, kept pasture-based mombaça grass (Panicum maximum) given ad libitum access water mineral supplementation. animals (n = 15) gynecologically examined uterine consistency evaluated rectal palpation same operator using an ultrasound system (SonoScape®) with linear transducer, operating frequency ranging 10 Mhz. tone classified grades 1 4 subjected induction. subjective perfusion day endometrium. determination progesterone concentration performed direct chemiluminescence assay. arcsine (√P/100) transformation applied percentage data, results expressed as mean (.) ± standard error (SEM). Further, assumptions normality homoscedasticity verified, respectively, Shapiro-Wilk Lilliefors tests. Regarding parametric non-parametric variables, applied, analysis variance (ANOVA) followed Tukey’s test, Kruskal-Wallis test Dunn’s test. Pearson’s correlation coefficient relationship parameters. statistical program SPSS 16.0 perform over-mentioned analyses, p-value < 0.05 taken significant. Corpus perfusion, evaluation methods, higher pregnant (P 0.05). methods positively correlated themselves well There no significant difference groups considering tonus > 0.05).Discussion: Mares that later became showed outcome It can be supported both values mares. Based results, concluded accurate tool determine vascularization, whether or methods. Also, efficient parameter predict ability maintain